Chitinase from Viscous Tissue
نویسندگان
چکیده
Three inherent acidic chitinases were purified from yam aerial tuber by the application of quaternary ammonium ion detergent. For remoyal of the mucilage in the crude extract of aerial tuber, the effeets of various quaternary ammonium ion detergent were compared. Cetylpyridinium chloride effectiyely remoyed the mucilage and this method proyed to provide high recoyery of chitinase actiyity. Three acidic chitinase isoforms were purified by anion-exchange chromategraphy, gel-filtration chromatography, and anion-exchange HPLC from the sllpernatant of cetylpyridinium chloride treatment. The three purified acidic chitinases had the molecular masses of 23, 25, and 31 kDa and proyed to be class III, class III, and class
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